Hydrogen bonds indicated by dashed lines. (A) The low-activity 3DVO structure shown with carbon atoms in gray overlaid with the high-activity ROO filament cryo-EM structure shown with carbon atoms in magenta. Preservation of contacts between SgrAI and the primary recognition sequence in DNA responsible for sequence specific recognition. Stereo views showing ( A ) a section of the ROO, ( B ) an alpha helix (residues 90–122), ( C ) the active site, with bound Mg 2+ ions (green), and ( D ) segmented density of the PC DNA.įigure S3. Correspondence between cryo-EM map and atomic model. ( G ) segmented DBD map colored by local resolution with ( H ) the corresponding atomic model.įigure S2. ( F ) FSC curves showing half-map resolution (red) and map-to-model resolution (green), both indicating a value of ~3.5 Å.3D-FSC(Tan et al., 2017) isosurfaces are displayed at a threshold of 0.5 within the inset for two perpendicular planar views. ( E ) Reconstruction from D colored by local resolution, with the full map at left and a cutaway of the center of the filament at right. In both C and D, a single DBD is highlighted in yellow. ![]() ( D ) High-resolution refinement of class 1 from C. ( C ) Reconstructed maps after asymmetric 3D classification showing a class resembling SgrAI (class 1) and two classes with ill-defined features. Cryo-EM data, reconstruction and validation.
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